Zymomonas

Zymomonas mobilis is a bacterium belonging to the genus Zymomonas. It is notable for its bioethanol-producing capabilities, which surpass yeast in some aspects. It was originally isolated from alcoholic beverages like the African palm wine, the Mexican pulque, and also as a contaminant of cider and beer in European countries.

Z. mobilis degrades sugars to pyruvate using the Entner-Doudoroff pathway. The pyruvate is then fermented to produce ethanol and carbon dioxide as the only products (analogous to yeast).

The advantages of Z. mobilis over S. cerevisiae with respect to producing bioethanol:

higher sugar uptake and ethanol yield (up to 2.5 times higher),[1]

lower biomass production,

higher ethanol tolerance up to 16% (v/v),[2]

does not require controlled addition of oxygen during the fermentation,

amenability to genetic manipulations.

However, in spite of these attractive advantages, several factors prevent the commercial usage of Z. mobilis in cellulosic ethanol production. The foremost hurdle is that its substrate range is limited to glucose, fructose and sucrose. Wild-type Z. mobilis cannot ferment C5 sugars like xylose and arabinose which are important components of lignocellulosic hydrolysates. Unlike E. coli and yeast, Z. mobilis cannot tolerate toxic inhibitors present in lignocellulosic hydrolysates such as acetic acid and various phenolic compounds.[3] Concentration of acetic acid in lignocellulosic hydrolysates can be as high as 1.5% (w/v), which is well above the tolerance threshold of Z. mobilis.

Several attempts have been made to engineer Z. mobilis to overcome its inherent deficiencies. National Renewable Energy Laboratory (NREL), USA has made significant contributions in expanding its substrate range to include C5 sugars like xylose and arabinose.[4],[5] Acetic acid resistant strains of Z. mobilis have been developed by rational metabolic engineering efforts, mutagenesis techniques [6] or adaptive mutation.[7],[8] However, when these engineered strains metabolize mixed sugars in presence of inhibitors, the yield and productivity are much lower, thus preventing their industrial application.

An extensive adaptation process was used to improve xylose fermentation in Z. mobilis.[9] Interestingly, by adapting a strain in a high concentration of xylose, significant alterations of metabolism occurred. One noticeable change was reduced levels of xylitol, a byproduct of xylose fermentation which can inhibit the strain’s xylose metabolism. One of the reasons for lower xylitol production was mutation in a putative gene encoding for an aldo-keto reductase that catalyzes the reduction of xylose to xylitol.[10],[11]

An interesting characteristic of Z. mobilis is that its plasma membrane

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